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Acid Fast stain |
| Test |
Acid Fast stain |
| Indication |
The staining is used to detect the presence of mycobacteria, monitor therapy against mycobacterium, efective diagnosis of mycobacterial infection.
|
| Physiology |
Acid fast bacteria are surrounded by a waxy envelope that is resistant to destaining by acid alcohol. Heat, prolonged exposure peneterates the cell wall. The stained bacteria is resistant to decolorization with acid alcohol.
|
| Normal Range |
No acid fast bacteria. Positive smears are quantitated and reported as 1+ (3-9 bacteria in entire smear), 2+ (>10 in entire smear), or 3+ (1 or more bacilli per field) acid fast bacteria seen.
|
| Sample |
Fluid, pus, or abscess material
|
| Test Method |
Acid fast stain - Ziehl-Neelsen or fluorochrome stain
|
| Related Tests |
Mycobacteria by DNA probe, Skin Test, Tuberculosis.
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